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NEUROGENOMICS
ROBERTO GIOVANNONI
Academic year2022/23
CourseNEUROSCIENCE
Code418EE
Credits6
PeriodSemester 1
LanguageEnglish

ModulesAreaTypeHoursTeacher(s)
NEUROGENOMICSBIO/18,BIO/09LEZIONI56
ROBERTO GIOVANNONI unimap
ENRICA STRETTOI unimap
Obiettivi di apprendimento
Learning outcomes
Conoscenze

 

 

Knowledge

Students of Neurogenomics course will be provided with robust basis on understanding the functional analysis and molecular mechanisms of the genes and the genomes related to the physiology and pathophysiology of the nervous system.

Assessment criteria of knowledge

Knowledge will be assessed by oral interview

Skills

Students will acquire the skills to analyze and discuss findings related to genetic/genomic studies in the field of neurophysiology and neuropathology. Students will also acquire the ability to design studies related to neurogenetic disorders by applying neurogenomics technologies and approaches.

Assessment criteria of skills

The skills acquired by the students will be assessed by means of oral interviews.

Behaviors

The student will be able to discriminate the genetic contribution from other etiopathological factors in complex multifactorial disorders of the nervous system. The student will be able to identify the advantages and limitations of experimental and clinical strategies aimed to correct defective genes (gene therapy, cell therapy, etc.)

Assessment criteria of behaviors

The behaviour acquired by the students will be assessed by means of an oral interview.

Prerequisites

Genetics as from the previous first-level degree of the admitted students.

Teaching methods

Classroom lectures, seminars, laboratory findings discussion.

Syllabus

“NeuroGenetics and NeuroGenomics” (Prof. Giovannoni)

The “NeuroGenetics and NeuroGenomics” part of the “Neurogenomics” course will focus on the functional and molecular analyses of the genes' and genome's work with regards to those elements and technologies relevant for neuronal physiology and pathology.

The following topics will be discussed by means of classroom lectures and seminars as well as interpretation and discussion of laboratory findings with the students:

  • Genetics and genomics in neuroscience: introduction, field of applications and perspectives in the context of neurology and neurosciences.
  • From the "monogenic disease" concept to the multi-gene and genome contribution in hereditary neurodegenerative disorders, the case of triplet repeat expansion diseases: molecular mechanism of mutation and its effects on the disease course; contribution of other genes in the genome in the disease onset and progression; molecular genetics of the “non-cell autonomous effect” in triplet repeat expansion disorders and the molecular role of glia in the pathogenesis and disease progression
  • The molecular complexity of CNS cancer, how the genetics and the genomics contribute to the definition of cells, mutations heterogeneity, drug resistance and tumor evolution: cues from single-cell genetic and genomic analyses in brain cancer 
  • Laboratory activities: Genetic tools for studying functions and dysfunctions of the central nervous From recombinase-mediated genetic editing (i.e. BRAINBOW) to CRISPR/Cas9 technique (i.e. ORANGE technology). The “Druggable Genome” strategy for uncurable neurogenetic diseases, Searching for uncharacterized genes with potential therapeutic impact. Use of Information aggregation systems and display for in-depth biomedical data (OMIM; GeneCards; OpenTargets; Pharos)

“Physiopathology and treatments of CNS genetic diseases” (Dr. Strettoi)

The “Physiopathology and treatments of CNS genetic diseases” part of the “Neurogenomics” course will focus on various diseases of the CNS with underlying genetic defects to illustrate genotype-phenotype correlations and experimental and clinical stage approaches to improve the disease outcome. The visual system will be often used as a paradigm to illustrate concepts and tools to which other genetic diseases of the CNS will be compared to extract general notions. Lectures involving specific CNS organs or areas (i.e. the callosal body; motor areas pertinent to the mirror system; etc.) will be introduced by summaries of the main anatomical organization, relevant morphological features, basic nomenclature and position in the brain as well as fundamentals of physiology. Indications for usage of web-based tools to navigate the brain will be provided. Various topics will comprise an experimental part illustrating modern methodological tools for the study of the CNS. Navigation and search of clinicaltrials.gov for the genetic diseases under study will be performed.

Specifically, the lectures will treat the following topics:

  1. General anatomy of the eye and the retina. Fundamental nomenclature. Inherited photoreceptor degenerations. From mutations to phenotype: known and (many) unknown cellular pathways to photoreceptor death starting from hundreds of mutations in 66 different genes. Retinitis Pigmentosa. The examples of rhodopsin and phosphodiesterase mutations. Retinal Pigment Epithelium, visual cycle and Leber Congenital Amaurosis. High photoreceptor efficiency and great genetic vulnerability.
  2. Ocular gene therapy from bench to bedside. From RPE65 mutations to commercially available Luxturna®. Ongoing gene therapy and open clinical trials for Retinitis Pigmentosa, Leber Congenital Amaurosis, Achromatopsia. Subretinal injections, intravitreal injections, ocular and global immune responses. The immune response in the CNS as a limiting factor for drug (or viral vectors; or protein) delivery. The Blood brain barrier: consequences for pharmacological treatment and gene therapy to access to the brain. Ebola and Zika in the eye.
  3. Viral Vectors for ocular gene therapy; limitations, targeting of specific cells. The undisclosed issue of inflammatory response to capsids. The problem and diverse approaches for correcting gain-of function mutations. Optogenetics to restore light sensitivity: state of the art of cell- specific promoters and extension to other genetic diseases. How to search and understand clinicaltrials.gov for clinical trials.
  4. Alternative approaches to neuronal rescue: inhibition of apoptosis mediated by ceramide using small molecules. Manipulations of the environment to promote retinal rescue (environmental enrichment: definition, effects). Neurotrophins as neuroprotectants. Prostheses: Argus II and epiretinal prostheses. Subretinal approach. Expected recovery of vision. The cochlear implant, similarities and differences with retinal prosthesis. The artificial nose: applications.
  5. Alzheimer and Parkinson diseases. Definition, main symptoms and incidence of genetic cases. Examples of rare genetic patients of a given pathology (i.e. Parkinson’s disease) as guidance for the discovery of the mechanisms of these diseases. Latest discoveries on multiple roles of Tau (including the nuclear access). Antibody therapy to target specific cellular functions and localization.
  6. Protein targeting in neurodegenerative diseases by antibody design. The case of Alzheimer, alpha-beta amyloid accumulation and employment of conformational specific antibodies. In vivo targeting of intracellular organelles.
  7. Amyotrophic Lateral Sclerosis (SLA). Definition. Symptoms. Genetic cases. The four major genes involved (SOD1, TDP-43, FUS, C9orf72).
  8. Cell therapy for neurodegenerative diseases of genetic origin. Organoids as recent tools for cell-based repair therapy and diagnosis of genetic defects. Brain organoid. The brain in a dish. The eye in a dish. Experimental advantages, perspectives and limitations.
  9. Connecting two parts of the brain. The callosal body: the largest commissural organ of myelinated fibers in the CNS. Organization and function. Complete Agenesis of the Callosal Body, pathophysiology and examples of underlying genetic defects. Mutations in Deleted in Colorectal Cancer gene and the multiple functions of the DCC protein.
  10. Regeneration and lack of regeneration in mammalian central and peripheral myelin; organization and molecular composition. Nogo, NgR, MAG. Inhibition of regeneration due to central myelin determinants. Reaction to injury in central and peripheral nerves. Dorsal root ganglia to study central and peripheral myelin effects in a single neuron. Possible involvement of mutations of myelin inhibitor genes in schizophrenia.
  11. Manipulation of regenerative capacities of the CNS by interference with the microenvironment: peripheral nerve grafts, employment of olfactory ensheating glia, electrical stimulation. Bedside approaches.
  12. Mirror neurons and mirror system in monkeys. Localization, basic physiological properties, possible implications in empathy and autism. Mirror system in birds. Correlation with articulated language. Mirror system dysfunctions in mammalians. Possible genetic causes of autism. The TRIO gene and its effects on the development and regulation of the strength of connections between brain cells. Mutations in TRIO.
Bibliography

Bibliography

1) Textbooks (suggested):

"Genetica molecolare umana ", by Tom Strachan & Andrew P. Read (Zanichelli) "Introduzione alla Genomica", by Greg Gibson & Spencer Muse (Zanichelli)

"Principles of Neural Science", by Eric R. Kandel, J.H. Schwartz and other Authors (5th edition)

"Applied Neurogenomics" Neuromethods series, Volume 97, by Kewal K. Jain (Humana Press)

2) Reviews and selected papers will be discussed and provided to the students via the dedicated e-learning website of the course

Assessment methods

Oral interview

Updated: 19/08/2022 22:11