The course will introduce to imaging techniques in the bio-medical field, from the basic concepts to the description of some advanced clinical applications. The topics will cover X-ray images (analogic and digital radiography), computed tomography (CT), Nuclear Magnetic Resonance (with imaging and spectroscopy applications), positron emission tomography (PET). The principles of optical imaging (fluorescence and bioluminescence), Cherenkov light imaging (CLI) and ultrasound and photoacoustic imaging will be also discussed.