MOLECULAR BIOPHYSICS

Code 422BB
Credits 5

Learning outcomes

By participating in the classes, the student will deepen their knowledge in the field of biophysics by performing research on various biological objects, from single molecules, through subcellular complexes and structures, to the structures of living matter using methodology and physics methods. It will be an opportunity to understand the basics of many advanced research techniques and take part in experiments performed using them. Familiarize yourself with, among others with the following research methods:
1. Spectroscopy and fluorescence microscopy used to observe the structure and follow cell life processes.
2. Multidimensional nuclear magnetic resonance (NMR) in imaging of tissue structure and observation of cellular changes.
3) Atomic force microscopy (AFM) in the study of individual molecules, forces of interaction between them and the structure of molecular and cellular systems as well as characteristics of their mechanical (viscoleastic) properties.
4) Microscale Raman spectroscopy - Raman mapping and surface enhanced Raman spectroscopy (SERS). 5) Electron cryomicroscopy of single molecules and molecular systems.
6) Mass spectrometry in the study of the atomic and molecular composition of substances and tissues (ToF-SIMS).
7) Analytical centrifugation.
8) Theoretical methods for modeling the structure, spectra and properties of molecules and their systems - the use of molecular dynamics and ab-initio modeling methods.