Analytical methods to study biological prerations

Code 431EE
Credits 3

Learning outcomes

The course, lasting 26 hours (14 hours of frontal lessons- 2CFU + 12 hours of exercises-1 CFU), is proposed to introduce students to several fundamental techniques in biochemistry, molecular biology and physiology. Methods to study biological samples (tissues or cell cultures)will be treated, both in terms of theory and practice to analyze products of lipid and glucose metabolism. Principal techniques will be direct to protein and lipids study (western blot, ELISA, HPLC), cell metabolism products (PCR, rt-PCR, real time qPCR), histological analysis of tissues (optic and fluorescence microscopy).