ECTS - University of Pisa
Scheda programma d'esame
ANIMAL MODELS AND TRANSGENIC ORGANISMS
MASSIMO PASQUALETTI
Academic year2016/17
CourseMOLECULAR AND CELLULAR BIOLOGY
Code368EE
Credits6
PeriodSemester 2
LanguageItalian

ModulesAreaTypeHoursTeacher(s)
ANIMALI MODELLO E ORGANISMI TRANSGENICIBIO/06LEZIONI56
MASSIMO PASQUALETTI unimap
Programma non disponibile nella lingua selezionata
Learning outcomes
Knowledge
The student who completes the course successfully will be able to demonstrate a solid knowledge of the main issues related to the principles of genome manipulation with particular attention to the mouse molecular genetics. He/she will acquire knowledge of the principles of transgenesis for the study of gene function and the generation of animal models for the study of human disease. Finally, he/she will receive advanced training for the planning of specific experimental approaches allowing him/her to face specific biological questions.
Assessment criteria of knowledge
- The student's ability to explain correctly the main topics presented during the course will be assessed. - The student will be assessed on his/her demonstrated ability to discuss the main course contents using the appropriate terminology. - The student will be asked to demonstrate the ability to simulate an experimental scheme for the generation of a transgenic line in order to answer to specific biological questions.

Methods:

  • Final oral exam

Further information:
The final evaluation is based on the final oral exam, though participation actively in discussion and showing interest during classes represent a positive feedback of the student demonstrating re-elaboration of the topic treated and learning.

Teaching methods

Delivery: face to face

Learning activities:

  • attending lectures
  • participation in discussions
  • Laboratory work

Attendance: Advised

Teaching methods:

  • Lectures
  • Seminar
  • Task-based learning/problem-based learning/inquiry-based learning
  • laboratory
Syllabus
The course provides an introduction to different animal models commonly used in a research laboratory focusing on the technologies available to manipulate their genomes. The course will introduce to the different strategies used to "solve" biotechnological specific questions. In particular experimental strategies for the generation of bioreactors with pharmacological applications, for the study if the gene function in vivo, and for the generation of animal models for human diseases and the studies of the etiological origin will be shown.
Bibliography
Manipulating the Mouse Embryo: A Laboratory Manual. By Andras Nagy, Samuel Lunenfeld Research Institute; Marina Gertsenstein, Samuel Lunenfeld Research Institute; Kristina Vintersten, European Molecular Biology Laboratory; Richard Behringer, University of Texas M.D. Anderson Cancer Center Jaenisch R. Nuclear cloning and direct reprogramming: the long and the short path to Stockholm. Cell Stem Cell. 2012 Dec 7;11(6):744-7. doi: 10.1016/j.stem.2012.11.005. Mak TW. Gene targeting in embryonic stem cells scores a knockout in Stockholm. Cell. 2007 Dec 14;131(6):1027-31. Wu S, Ying G, Wu Q, Capecchi MR. A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. Nat Protoc. 2008;3(6):1056-76. PASQUALETTI MASSIMO, PACINI GIULIA,Perché Mario R. Capecchi, Martin J. Evans e Oliver Smithies hanno ricevuto il Premio Nobel 2007 per la fisiologia e la medicina? in Perché Nobel?,pp 129, 143,tot.pag. 15,2009
Updated: 14/11/2016 17:27