Academic year2016/17
CourseMOLECULAR AND CELLULAR BIOLOGY
Code183EE
Credits6
PeriodSemester 2
LanguageItalian
| Modules | Area | Type | Hours | Teacher(s) |
| BIOLOGIA MOLECOLARE 2 | BIO/11 | LEZIONI | 48 | |
Programma non disponibile nella lingua selezionata
Knowledge
The student who successfully completes the course will be able to demonstrate a solid knowledge of the main mechanisms involved in the control of gene expression. He or she will acquire the abiity to critically analyze scientific papers related to molecular biology issues.Furthermore, the student will be aware of basic and advanced molecular biology techniques. Finally, he or she will learn to design appropriate strategies to approach specific problems in molecular biology.
Assessment criteria of knowledge
The student will be assessed on his/her demonstrated ability to discuss the main course contents using the appropriate terminology. During the oral exam the student must also demonstrate the ability to approach a circumscribed research problem describing the appropriate techniques/technologies to be used.
Methods:
Teaching methods
Delivery: face to face
Learning activities:
Attendance: Advised
Teaching methods:
Syllabus
The course analyzes the main events occurring in the control of gene expression at the transcriptional, post-transcriptional, translational and post-translational levels in eukariotic cells. Particular attention is dedicated to experimental strategies aimed at dissecting molecular mechanisms. This is achieved in the first part of the course by acquiring knowledge on techniques and methodologies used in the study of proteins and nucleic acids (including genomic and transcriptomic studies), as well as protein-protein and protein-nucleic acid interactions and, in the second part of the course, by critically reading specific articles.
Bibliography
Textbooks:
- Lewin – Il Gene VIII– Zanichelli
- Lodish, Berk, Zipursky, Matsudaira, Baltimore, Darnell - “Biologia Molecolare
della Cellula” –Zanichelli
Specific article analyzed during the course include:
The ENCODE Project Consortium Nature, 489, 57-74, 2012 An integrated
encyclopedia of DNA elements in the human genome.
Eulalio et al. Nature 492: 376-381, 2012. Functional screening identifies miRNAs
inducing cardiac regeneration.
Gargiulo et al., Dev Cell. 16:466-481, 2009. NA-Seq: a discovery tool for the analysis of
chromatin structure and dynamics during differentiation.
Gargiulo e Minucci Int J Biochem Cell Biol. 41:127-35, 2009. Epigenomic profiling of
cancer cells.
Hong et al., Science 321:1314, 2008. Shadow enhancers as a source of evolutionary
novelty.
Kim et al., Nat Rev Mol Cell Biol,10, 126?139, 2009. Biogenesis of small RNAs in animals
Martin et al., Cell 136, 719–730, 2009. mRNA Localization: Gene expression in the
spatial dimension
Mili et al., Nature 453, 115-119, 2008. Genome-wide screen reveals APC-associated
RNAs enriched in cell protrusions
Pennisi Science 337, 1159-1161, 2012. ENCODE Project Writes Eulogy For Junk DNA.
Perry et al., Curr Biol. 20:1562-1567, 2010. Shadow enhancers foster robustness of
Drosophila gastrulation.
Prabhakar et al., Science 321:1346-1350, 2008. Human-specific gain of function in a
developmental enhancer.
Semenza, Physiology 24: 97-106, 2008. Regulation of oxygen homeostasis by hypoxiainducible
Factor 1.
Wilhelm et al., J. Cell Biol. 148, 427-439, 2000. Isolation of a Ribonucleoprotein
Complex involved in mRNA localization in Drosophila oocytes
Updated: 14/11/2016 17:27
