ECTS - University of Pisa
The student who successfully completes the course will have the ability to apply several DNA manipulation techniques for biotechnological exploitation of microbial cells, virus, and eukaryotic cells; will be able to demonstrate a solid knowledge of physiology of microbial cells, of their metabolic flexibility, and growth kinetis: will be able to demonstrate advanced knowledge of recombination mechanisms occurring in microbial cells, yeast included, to delineate the use of viruses,plasmids,and trasposons as genetic vectors for DNA manipulation studies; will be aware of producing molecular protocols for identify bacterial species and to generate recombinant cells for biotechnological purposes.
- During the oral exam the student must be able to demonstrate his/her knowledge of the course material and be able to discuss the reading matter thoughtfully and with propriety of expression. - The student must demonstrate the ability to put into practice, with critical awareness, the laboratory activities carried out during the course.
Methods:
Further information:
Final oral exam (70%) Laboratory practical (30%)
Delivery: face to face
Learning activities:
Attendance: Not mandatory
Teaching methods:
(Molecular Biology)The course covers the basic properties of DNA, RNA and proteins involved in regulatory mechanisms of replication, repair, transcription of genomes; describes critically the basic principles of the DNA manipulation techniques, their biotechnological exploitation and application to fundamental research. (Microbiology) The course is focused on the knowledge of morphological–functional and structural-molecular properties of Eubacteria, Archea and Yeast, in view of their potential biotechnological use. Describes the physiology of microbial cells, their metabolic flexibility, growth kinetics and basic recombination mechanisms enabling the correct use of viruses, plasmids, and trasposons as genetic vectors. The course includes the principal human microbial pathogens and their interaction with the host.
(Molecular Biology Watson, T. A. Baker, S. P. Bell, A. Gann, M. Levine, R. Losick - “Biologia molecolare del gene” 6a edizione. Zanichelli 2009; G. Dehò E.Galli. Il mondo dei microrganismi, Casa Editrice Ambrosiana. 2011 Recomended reading includes the followings: slides (power point presentations) prepared by the teacher, which include all issues oh the course.
Acquisition of different molecular methodologies useful for the identification of microbial species; Ability to generate microbial clones expressing etherologous genes.
