ECTS - University of Pisa
Scheda programma d'esame
BIOCHEMISTRY
ANTONELLA DEL CORSO
Academic year2016/17
CourseBIOTECHNOLOGY
Code113EE
Credits9
PeriodSemester 1 & 2
LanguageItalian

ModulesAreaTypeHoursTeacher(s)
BIOCHIMICABIO/10LEZIONI80
ANTONELLA DEL CORSO unimap
Programma non disponibile nella lingua selezionata
Learning outcomes
Knowledge
The student who successfully completes the course will be able to demonstrate a solid knowledge in Biochemistry starting from basic principles of general biochemistry to main metabolic pathways. The student will be able to correlate the knowledge of general features of proteins and enzymes with their cellular/organ function and regulation. The student will perform simple experimental measurements in a biochemistry laboratory and will be able to discuss and make calculations on the acquired data.
Assessment criteria of knowledge
- During the oral exam the student must be able to demonstrate his/her knowledge of the course material and be able to discuss different subjects using the appropriate terminology. - The student must demonstrate the ability to analyze, with critical awareness, the practical activity performed in the Laboratory.

Methods:

  • Final oral exam
  • Laboratory report
Teaching methods

Delivery: face to face

Learning activities:

  • attending lectures
  • Laboratory work

Attendance: Advised

Teaching methods:

  • Lectures
  • laboratory
Syllabus
Features and properties of relevant biomolecules; protein structure and function; basic principles of enzyme kinetics and enzyme regulation; anabolism and catabolism; glycolysis and gluconeogenesis; pentose phospates shunt;glycogen homeostasis; Krebs cycle; electrons transport and oxidative phosphorylation; fatty acids metabolism: synthesis and degradation.
Bibliography
ISBN 9788808064035 David L Nelson, Michael M Cox I principi di biochimica di Lehninger Quinta edizione – 2010 Zanichelli or/and ISBN 978880817544-1 Donald Voet, Judith G Voet, Charlotte W Pratt Fondamenti di biochimica terza edizione -2007 - Zanichelli or/and ISBN 9788808198730 Jeremy Berg, John Tiymoczko, Lubert Stryer Biochimica Settima edizione 2012 Zanichelli
Work placement
Problem solving on equilibrium reactions; calculation of isoelectric point of peptides. Preparation of buffer solutions. Determination of protein content in biological samples. Spectrophotometric determination of enzyme activity. Determination of kinetic parameters for an enzymatic reaction. Effect of pH on enzyme activity.
Updated: 14/11/2016 17:27