ECTS - University of Pisa
| Modules | Area | Type | Hours | Teacher(s) | |
| OSSERVAZIONE MICROSCOPICA DEI TESSUTI UMANI | BIO/17 | LEZIONI | 28 |
|
The student who successfully completes the course will have the ability to identify specimens of the human tissues. He will be able to demonstrate a solid knowledge of the most relevant histological techniques beginning from the sample withdrawal, to fixation treatment, ending with sectioning and staining. Beside this the student will be aware of the modern immunohistochemical pocedures employed for molecules detection inside the cells and tissues of the human body.
- In the written exam the student must demonstrate his/her knowledge of the course material and to organise an effective and correctly written reply. The student must demonstrate the ability to put into practice and to execute, with critical awareness, the activities illustrated or carried out under the guidance of the teacher during the course.
Methods:
Delivery: face to face
Learning activities:
Attendance: Advised
Teaching methods:
specimen fixation. Embedding methods. Cryostatic sections. Staining techniques in histology. histochemistry staining of the constituents of tissues Principles and main applications of enzyme histochemistry. Immunohistochemistry, immunofluorescence, immunocytochemistry. Monoclonal antibodies and their application in histology. In situ hybridization, in situ PCR: histoautoradiographic techniques. Electron microscopy application of histochemistry and immunohistochemistry. Application of histochemistry and immunohistochemistry to in vitro cultured cells and tissues.Laboratory: light microscopic observation and identification of histological samples of human tissues processed by histological and histochemical methods: surface epithelia, glandular epithelia, connective tissue, cartilage tissue, bone tissue, blood, nervous tissue, muscular tissue).
MONESI; Istologia, Ed. Piccin nuova edizione 2012
