Scheda programma d'esame
MICROBIOLOGY AND PUBLIC HEALTH
ARIANNA TAVANTI
Academic year2022/23
CourseBIOTECHNOLOGIES AND APPLIED ARTIFICIAL INTELLIGENCE FOR HEALTH
Code022FE
Credits12
PeriodSemester 2
LanguageEnglish

ModulesAreaTypeHoursTeacher(s)
HEALTH RISK ASSESSMENTMED/42LEZIONI48
ANNALAURA CARDUCCI unimap
MARCO VERANI unimap
MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGYBIO/19LEZIONI48
DARIA BOTTAI unimap
ARIANNA TAVANTI unimap
Obiettivi di apprendimento
Learning outcomes
Conoscenze

VALUTAZIONE DEL RISCHIO PER LA SALUTE

Gli studenti acquisiranno una conoscenza di base dell'epidemiologia e della prevenzione delle malattie, nonché della metodologia di analisi del rischio sulla salute. 

Knowledge

HEALTH RISK ASSESSMENT

The students will acquire a basic knowledge of epidemiology and prevention of diseases, as well as of health risk analysis methodology.  

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

The student who successfully completes the course will be able to demonstrate a solid knowledge of cellular and molecular organization of the main groups of Eubacteria and Archea, Virus and Yeast; He/She will gain a general knowledge of microbial physiology, genome organization, and of the main methods leading to genetic manipulation for biotechnological purposes, as well as of virulence/pathogenicity factors of microorganisms and how they interact with the host.

Modalità di verifica delle conoscenze

Per l'accertamento delle conoscenze sarà svolto un esame orale finale.

 

Assessment criteria of knowledge

HEALTH RISK ASSESSMENT

The student will be assessed with a final oral exam.

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

The student will be assessed with a final oral exam.

Capacità

Lo studente sarà in grado di pianificare piani di valutazione del rischio per gli ambienti di vita e di lavoro, attraverso le sue fasi fondamentali: identificazione e caratterizzazione del pericolo, valutazione dell'esposizione e valutazione del rischio, utilizzando metodi tradizionali e innovativi.

Skills

HEALTH RISK ASSESSMENT

The student will be able to plan the risk assessment for life and working settings, through its basic phases: hazard identification and characterization, exposure assessment and risk evaluation, using traditional and innovative methods.

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

The student will be able to understand and discuss ther structure of microbial cells, and the basics of genetic manipulation of microorganisms for biotechnological purposes

Modalità di verifica delle capacità

Durante l'esame orale lo studente sarà stimolato a risolvere problematiche legate alla valutazione del rischio per la salute con le basi fornite da corso.

Assessment criteria of skills

HEALTH RISK ASSESSMENT

During the oral exam students will be encouraged to solve health risk assessment problems by the course provided methods

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

The student will be assessed on his/her demonstrated ability to discuss the main course contents using the appropriate terminology. 

Comportamenti

Lo studente potrà acquisire competenze nell'organizzazione di un lavoro di analisi di rischio ed interpretazione dati.

 

Behaviors

HEALTH RISK ASSESSMENT

Students will acquire skills in organizing in health risk analysis and data interpretation

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

Students will acquire and/or develop an awareness of microbial cell organization and physiologyas well as of microbial biotechnology

Modalità di verifica dei comportamenti

Durante l'esame orale lo studente sarà valutatato nellle metodiche utilizzate per risolvere problematiche legate alla valutazione del rischio per la salute.

Assessment criteria of behaviors

HEALTH RISK ASSESSMENT

During the oral exam students will be evaluated relating the methodology used to solve health risk assessment problems.

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

During the oral exam students will be evaluated based on the ability to appropiately discuss the topics of the course 

Prerequisiti (conoscenze iniziali)

Conoscenze di base legate alla biologia

Prerequisites

HEALTH RISK ASSESSMENT

Knowledge related to biology 

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

Basic Knowledge of citology

Indicazioni metodologiche

Lezioni frontali con ausilio di slides. Sito elearning usato per caricamento slides. Comunicazione con gli studenti tramite ricevimento e posta elettronica.

Teaching methods

HEALTH RISK ASSESSMENT

Lectures with slides. Site elearning used to upload slides. Communication with students through email.

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY 

Lectures with slides and active discussion. Slides will be uploaded on the moodle e-learning website.

Programma (contenuti dell'insegnamento)

Basi per la valutazione del rischio per la salute

Metodologia epidemiologica

Principi generali di epidemiologia

Epidemiologia e prevenzione delle malattie infettive

Epidemiologia e prevenzione delle malattie non infettive.

 

Analisi del rischio per la salute pubblica

 

Valutazione del rischio per la salute (identificazione del pericolo, caratterizzazione del pericolo, valutazione dell'esposizione, caratterizzazione del rischio)

Gestione del rischio sanitario

Comunicazione del rischio sanitario

Applicazioni specifiche dell'analisi di rischio: Alimenti, matrici ambientali (acqua, aria interna ed esterna) e ambienti di lavoro

Applicazione dell'epidemiologia molecolare, dei sensori e dei biomarcatori e dell'informatica all'analisi del rischio in sanità pubblica

Syllabus

HEALTH RISK ASSESSMENT

Basis for the health risk assessment

Epidemiological methodology

General principles of epidemiology

Epidemiology and prevention of infectious diseases

Epidemiology and prevention of no communicable diseases.

Risk analysis  for public health

Health Risk Assessment (Hazard identification, Hazard characterization, Exposure assessment, Risk characterization)

Health risk management

Health risk communication 

Specific applications of risk analysis: Foods, Environmental matrices (water, indoor and outdoor air) and working settings 

Application of molecular epidemiology, of sensors and biomarkers and of information technology to risk analysis in public health

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY

Taxonomy and classification of bacteria. Eubacteria, Archaea and Eukaryotes. Characteristics of cocci and bacilli. Cell and colony morphology.
The bacterial cell wall, functions and general characteristics. Structure and organisation of peptidoglycan. Biosynthesis of murein: cytoplasmic phase, membrane phase and post-translocation in the extracellular compartment. Biogenesis of the bacterial wall. Structure and chemical composition of the cell wall in Gram-positive and Gram-negative bacteria.
Peptidoglycan, periplasm, teichoic, lipoteichoic and teicuronic acids in Gram positive bacteria. Periplasmic compartment, role of periplasmic proteins, outer membrane in Gram negative bacteria. The lipopolysaccharide, structure and composition. Biological effects of LPS.
Structure and function of porin proteins. Translocation and assembly of the molecules constituting the outer membrane (porins, lipoproteins and LPS). Peculiarities: cell wall of Mycobacteria and Chlamydia spp. The cell wall of the Archaeabacteria (features and different types). Antibiotics active on the bacterial cell wall and drug resistance.
The plasma membrane in Eubacteria and Archaea. Bacterial mesosomes. Antibiotics active on the cell membrane (Polymyxins, Lipopeptides, Polyenes) The protoplast: bacterial cytoplasm, cytoskeleton and ribosomes. Antibiotics active on protein synthesis. Cytoplasmic inclusion bodies: characteristics, nature and functions.
The capsule as a virulence factor. Methods to highlight its presence in the laboratory. The bacterial spore: life cycle of a spore-forming bacterium, peculiarities, structure and position of the spore. Sporulation stages. Spore structure. Activation, germination and outgrowth.
Organization of bacterial genomes. The nucleoid and plasmids.
Protein secretion in bacteria. The SRP systems. TAT secretion system. ABC Conveyors. "One-step" and "two-step" models of secretion. Type II-IX secretion systems: general characteristics and peculiarities. Type VII (or ESX) systems in mycobacteria: characteristics and functions.
The bacterial flagellum: structure, biosynthesis and rotation. Bacterial movement and taxis. Chemotaxis. Molecular mechanisms underlying chemotaxis.
.
Measurement of the turbidity of a bacterial culture, evaluation of bacterial growth through the measurement of the optical identity (indirect measurement); direct methods total count, viable cell count. Graphical representation of bacterial growth. Latency phase, exponential growth phase (generation time calculation), stationary and death phases.
Genetic variability in bacteria. The mutation. Homologous, site specific and illegitimate recombination. Horizontal gene transfer: tansformation, conjugation and transduction. Transformation: physiological conditions and molecular mechanisms responsible for the induction of the state of competence and the DNA uptake via tansformation.
Conjugation. The F plasmid of Escherichia coli as paradigm of conjugative plasmids . Main steps and variants of the conjugative process, and their evolutionary implication.
Phage-mediated DNA transduction. Morphological and structural characteristics of phages. Replicative cycle of virulent phages and lysogenic cycle of temperate phages. Generalized and specialized transduction. The fate of molecules acquired by horizontal gene transfer: outline of restriction/modification systems and CRSPR/Cas systems. General characteristics of transposons and their application in microbial biotechnologies.
Gene editing in bacteria: main strategies for selective bacterial gene inactivation or modification
Gene editing in bacteria part II: genetic circuits for the expression and release of target molecules by engineered bacteria.
Main approaches for the biocontainment of engineered microorganisms.
Host-parasite interaction. Commensalism and parasitism. Events associated with the onset of a disease: contagion, adhesion, invasion, infection and disease. Virulence factors and bacterial pathogenicity. The exotoxins. Exotoxins vs endotoxins.
Cytolytic toxins, A-B toxins. Examples of toxin with a complex biological action: diphtheria toxin. Mechanism of action of cholera toxin and neurotoxins (tetanus and botulinum) Bacillus anthracis exotoxin. Pathogenicity islands.
Microorganisms and their potential application in biosensing devices.
Fungal pathogens: yeast and molds. Structure and characterisitics of fungal cells.
Genetic manipulation of Yeast (disruption cassettes vs gene editing).
Animal viruses: structure, shape and size. Structure and composition of the capsid and envelope. Main phases of the replication cycle of an animal virus. Baltimore classification. Replicative strategies of viruses belonging to the different classes of Baltimore. Types of animal virus infection.
Vaccine development and innovative vaccine strategies.

Bibliografia e materiale didattico

Professione Igienista – Manuale dell’Igiene Ambientale e Territoriale – Gilli – Casa Editrice Ambrosiana

Bibliography

HEALTH RISK ASSESSMENT

Professione Igienista – Manuale dell’Igiene Ambientale e Territoriale – Gilli – Casa Editrice Ambrosiana

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY:

Microbe, 3rd Edition
Michele S. Swanson, Elizabeth A. Joyce, Rachel E. A. Horak
ISBN: 978-1-683-67372-9 | June 2022 | ASM Press | 752 Pages

Non-attending students info

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY 

The same program will be used to assess the knowledge of non attending students

Modalità d'esame

La prova orale consiste in un colloquio tra il candidato, il docente e altri collaboratori del docente titolare. La prova orale non è superata se il candidato mostrerà ripetutamente l'incapacità di conoscere correttamente le nozioni basilari del corso e di mettere in relazione parti del programma e tali nozioni per rispondere in modo corretto ad una domanda.  

 

 

Assessment methods

HEALTH RISK ASSESSMENT

The oral test consists of an interview between the candidate, the teacher and other collaborators of the lecturer. The oral test is not accepted if the candidate repeatedly show the inability to properly learn the basics of the course and put parts of the program report and these notions to respond correctly to a question.

MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY 

The oral exam will consists of a public interview between the candidate and the Lecurers.

 

Updated: 12/02/2023 17:56