CourseMOLECULAR BIOTECHNOLOGY
Code527EE
Credits3
PeriodSemester 2
LanguageEnglish
Modules | Area | Type | Hours | Teacher(s) | |
PLANT SYNTHETIC BIOLOGY | BIO/04 | LEZIONI | 24 |
|
The course aims at providing a description of the theoretical framework of synthetic biology and, subsequently, an overview of current applications of synthetic biology to plant science.
At the end of the course, students will possess knowledge of the general concepts of synthetic biology and of the state-of-the-art methodologies and techniques used for the design of novel biological components and gene circuits, or the re-design of existing biological systems. Moreover, students will gain in-depth awareness of the current applications of synthetic biology to plant science, thanks to the analysis of a wide range of examples from the literature presented during lectures.
Students will be assessed on (i) the acquisition of familiarity with general concepts of synthetic biology and (ii) their capacity to harness the theoretical and methodological resources available to plant biologists today to tackle open questions in the field of plant science adopting synthetic biology approaches.
The acquired knowledge will be subjected to three layers of evaluation:
- knowledge related to the lecture hours will be assessed with an intermediate written test;
- knowledge of the applicative perspectives of synthetic biology to plant science will be assayed through the individual presentation of a small proposal.
At the end of the course, students will enhance their ability to conceive, propose and present viable strategies that take advantage of state-of-the-art engineering technologies of plant, microbes and biological communities aimed at plant biotechnology, plant science and crop improvement.
Skills will be verified
- through the students’ capacity to analyze published case studies that employ synbio approaches, critically present them to the class and discuss the methods used and the significance of the described findings;
- through the development of a small applicative proposal, related to a topic proposed by the teacher, aimed at solving or describing a biological problem in the field of plant science by use of synthetic biology approaches.
Students are expected to be actively enrolled in the build-up of the interactive part of the course.
A curiosity-driven approach to the topics proposed by the teacher is, moreover, encouraged throughout the whole course.
Students’ behaviours will be evaluated according to their participation in problem solving and the attitude to express their critical thinking by raising questions.
Molecular biology, biochemistry, plant molecular physiology, genetics.
Basic bioinformatics knowledge.
Teaching hours will be organized into three different kinds of activities: lectures, flipped teaching and supervised problem-solving activities. Depending on the number of attendees, the second and third activity will be either individual or consisting of team works.
- Enabling technologies: DNA sequencing, gene synthesis, cloning, DNA assembly techniques.
- Synthetic expansion of the genetic code.
- Synthetic DNA circuits and logic gates.
- Protein engineering: high-throughput methods for the generation and selection of protein variants.
- Synthetic genomes and cells.
- Social and ethical issues concerning synthetic biology.
- Precision genome editing in plants.
- Design and expression of genetically encoded biosensors in plants.
- Plant metabolic engineering in plants: case studies.
- Selected relevant research papers and reviews from the scientific literature.
- Textbook: "Synthetic Biology: Tools and Applications”, 1st, Huimin Zhao. Academic Press. Amsterdam (The Netherlands) and Boston (Massachusetts), Elsevier. ISBN: 978-0-12-394430-6. 2013."
- Additional learning material shared by the lecturer on the e-learning platform.
Active enrollment is envisioned as part of the students’ evaluation, therefore attendance to the supervised activities and flipped teaching classes is mandatory.
The exam consists of three partial tasks, described in the previous section “Assessment criteria of knowledge" to be accomplished along the course. The final grade is given by the arithmetic average of the marks of the three tasks.
Students are invited to verify the existence of any preparatory requirements by consulting the Regulations of the Study Program relating to their year of enrollment. Exams taken in violation of the propaedeutic rules are invalid (Regolamento didattico d’Ateneo, art. 24, comma 3).
Up-to-date record of lessons and subject matters will be available on the lecturer’s web pages on Unimap (University of Pisa web site).
EXAM COMMITTEE
President: Beatrice Giuntoli
Members: Chiara Pucciariello, Riccardo Di Mambro
Deputy President: Chiara Pucciariello
Deputy Members: Andrea Andreucci, Noemi Violeta Poma Sajama